Elucidation of Secondary Metabolites from Thyme Oil and Comparing its Antimicrobial Potential with Conventional Antibiotics against Uropathogens
Archana Kulkarni1, Nasreen Jan2, and
1.Department of Microbiology, Dharampeth M.P.Deo Memorial Science College, Nagpur, MS, India
2.PGTD of Microbiology, RTM, Nagpur University, Nagpur,MS, India
Abstract—GC-MS analysis of thyme oil showed the presence of various secondary metabolites comprising 13 components representing approximately 99% of the oil. The major components were carvacrol, thymol, terpinene-4-ol. The antimicrobial activity of thyme oil was assessed by disc diffusion method against uropathogens like Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus vulgaris, P. mirabilis and Staphylococcus aureus. The MIC was determined by broth microdilution method. The ZOI and MIC (%) were in the range of 19±3 mm and 0.08% (v/v equivalent) for E. coli; 14±4 mm and 0.25% for S. aureus; 16±4 mm and 0.04% for K. pneumoniae; 18±3mm and 0.04% for Proteus mirabilis; 18±3 mm and 0.04% for Proteus vulgaris. Thyme oil showed low MIC values and high growth inhibition in comparison to 16 broad spectrum antibiotics tested. The result of the bioassay showed potent bactericidal properties of the oil.
Index terms—Thyme oil, secondary metabolites, carvacrol, terpinen-4-ol, uropathogens, bactericidal.
Cite: Archana Kulkarni, Nasreen Jan, and Seema Nimbarte, "Elucidation of Secondary Metabolites from Thyme Oil and Comparing its Antimicrobial Potential with Conventional Antibiotics against Uropathogens," Journal of Life Sciences and Technologies, Vol.1, No.1, pp.29-32, March 2013. doi: 10.12720/jolst.1.1.29-32