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Sequence Variation of CSLA Gene Responsible for the Synthesis of Glucomannan in Porang (Amorphophallus Muelleri Blume) Collected from Java, Indonesia

Estri Laras Arumingtyas and Arik Arubil Fatinah
Biology Department, Faculty of Science, Brawijaya University, Jl. Veteran, Malang, Indonesia 65145
Abstract—Porang (Amorphophallus muelleri Blume) collected from various geographical locations has been known to show variation on glucomannan content. Glucomannan is polysaccharide consisting hydrocoloid d-glucose and d-mannose which has high economical value. One of the most important genes responsible for the synthesis of glucomannan is cellulose synthase-like A (CSLA) glycosyltransferase. From previous study, CSLA gene was only found in the the flower spatha and bulb of only one sample, lead to the failure to detect it is effect to the glucomannan content. So, in this research, identification of CSLA gene was conducted using Polymerase Chain Reaction (PCR) technique with different primer, to detect the influence of CSLA gene to the glucomannan content of porang collected from varous part of Java Island, Indonesia. The result showed that glucomannan content between porang originating from different locations were not the same. This might be due to genetic and environmental factors. It was detected that the sequence of CSLA genes of porang from different location were different, the interaction of both factors are likely to cause differences in the glucomannan produced.

Index Terms—porang (amorphophallus muelleri blume), CSLA gene, glycosil transferase, glucomannan

Cite: Estri Laras Arumingtyas and Arik Arubil Fatinah, "Sequence Variation of CSLA Gene Responsible for the Synthesis of Glucomannan in Porang (Amorphophallus Muelleri Blume) Collected from Java, Indonesia," Journal of Life Sciences and Technologies, vol. 3, no. 1, pp. 7-10, June 2015. doi: 10.18178/jolst.3.1.7-10
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