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Effect of Soil Extract in Different Culture Medium for Marine Microalgae’s Biomass and Lipid Production in Biodiesel Production

Chee Loong Teo, Ani Idris, and Junaid Akhtar
Department of Bioprocess Engineering, Faculty of Chemical Engineering, c/o Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia, 81310, UTM Johor Bahru, Johor, Malaysia

Abstract—Optimization culture medium is a crucial factor that significantly affects the lipid production and specific growth rate of marine microalgae. The main purpose of this research was to optimize the marine microalgae’s growth in modified culture medium under the influences of soil extract in F/2 medium and Walne medium. Nannochloropsis sp. was successfully grown for 14 days at two difference of culture medium at the control environment 23±0.5 oC, pH 8±0.2 under a light intensity of 100 μmol m-2s-1. Growth rate of marine microalgae was observed by optical density method and lipid production investigated by Nile Red method. In this research, 7.0% of soil extracts concentrations in culture medium promote the growth rate and lipid production. At the end of the research, crude oil from marine microalgae was converted to biodiesel and analyzed by Gas Chromatography.

Index Terms—soil extract, culture medium, marine microalgae, biomass, lipid

Cite: Chee Loong Teo, Ani Idris, and Junaid Akhtar, "Effect of Soil Extract in Different Culture Medium for Marine Microalgae’s Biomass and Lipid Production in Biodiesel Production," Journal of Life Sciences and Technologies, vol. 4, no. 1, pp. 22-25, June 2016. doi: 10.18178/jolst.4.1.22-25
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